Double Stains


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Mitotic vs Proliferative
  • DAB Brown:
    PHH3
    Mitotic figures

  • Alk Phos Red:
    Ki-67
    Other proliferating cells

Tissue Type:
Tonsil
Magnification:
400x
CD61 + CD71
  • DAB Brown:
    CD61
    Megakaryocytes

  • Alk Phos Red:
    CD71
    Erythroid precursors

Tissue Type:
Bone marrow
Magnification:
400x
BCL6 + BCL2
  • DAB Brown:
    BCL6*
    Malignant follicular & germinal center cells

  • Alk Phos Red:
    BCL2
    Malignant follicular & interfollicular cells

Tissue Type:
Follicular lymphoma in situ
Magnification:
100x
*BCL6 is protected by U.S. patents 6,174,997 and
6,783,94 (Cancer Genetics, Inc.)
B-cell vs T-cell
  • DAB Brown:
    CD3
    T-cells

  • Alk Phos Red:
    CD20
    B-cells

Tissue Type:
Tonsil
Magnification:
100x
Lymphoma Monoclonality
  • DAB Brown:
    Kappa
    Plasma cells

  • Alk Phos Red:
    Lambda
    Plasma cells

Tissue Type:
Tonsil
Magnification:
400x
Ductal vs Lobular Breast Carcinoma
Tissue Type:
Lobular breast CA
Magnification:
200x
CK 20 + p53 + CD44
  • DAB Brown:
    CK 20
    Superficial epithelium
  • p53
    Nuclei

  • Alk Phos Red:
    CD44
    Basal epithelium

Tissue Type:
Urothelial dysplasia
Magnification:
200x
CK 5 + CK 14 + p63 + CK 8 & 18
  • DAB Brown:
    CK 5 + CK 14 + p63
    Myoepithelial cells

  • Alk Phos Red:
    CK 8 & 18
    Intraductal CA cells

Tissue Type:
Breast, intraductal carcinoma
Magnification:
100x
CD23 + CD5
  • DAB Brown:
    CD23
    Germinal center cells

  • Alk Phos Red:
    CD5
    Lymphocytes

Tissue Type:
Tonsil
Magnification:
200x
Benign vs Malignant Prostate
  • DAB Brown:
    CK 5, CK 14
    Prostate basal cells

  • Alk Phos Red:
    P504s
    Prostate cancer cells

Tissue Type:
Prostate CA
Magnification:
400x
Ki-67 + MART-1
  • DAB Brown:
    Ki-67
    Melanoma cells

  • Alk Phos Red:
    MART-1
    Melanoma cells

Tissue Type:
Malignant melanoma
Magnification:
400x
CD15 + CD30
  • DAB Brown:
    CD15
    Reed-Sternberg cells & neutrophils

  • Alk Phos Red:
    CD30
    Reed-Sternberg cells & neutrophils

Tissue Type:
Hodgkin's disease
Magnification:
400x
Benign vs Malignant Liver
  • DAB Brown:
    Glypican-3
    Hepatocellular carcinoma

  • Alk Phos Red:
    Hep-Par1
    Benign hepatocytes

Tissue Type:
Liver cancer
Magnification:
100x
CD14 + Myeloperoxidase
Tissue Type:
Bone marrow
Magnification:
400x
GCDFP-15 + Mammaglobin
Tissue Type:
Breast carcinoma
Magnification:
200x
In situ vs Invasive Breast Carcinoma
Tissue Type:
Breast carcinoma
Magnification:
100x

Antibodies sold separately.