BOB.1 (SP92) Rabbit Monoclonal Antibody
Specialties: Hematopathology
Oct-binding factor-1 (OBF1), also known as BOB.1, is a B-cell-specific coactivator which has been mapped to chromosome 11q23.1 Expression of BOB.1/OBF.1 is restricted largely to mature B-cells, with germinal center B-cells normally staining for BOB.1.2,3 Analyses of BOB.1/OBF.1 expression in a variety of established B-cell lines representing different stages of B-cell development has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immunoreactive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immunoreactivity within the Hodgkin and Reed-Sternberg cells.3-7 Expression of BOB.1/OBF.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.8-12 B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.11-12
- Junker S, et al. Assignment of the human gene for Oct-Binding Factor-1 (OBF1), a B-Cell-spefific coactivator of Octamer-Binding transcription Factors1 and 2, to 11q23.1 by somatic cell hybridization and in situ hybridization. Genomics. 1996; 33:143-5.
- Dabbs DJ. Diagnostic Immunohistochemistry, Third Edition. Saunders. 2006.
- Steimle-Grauer SA, et al. Expression patterns of transcription factors in progressively transformed germinal centers and hodgkin lymphoma. Virchows Arch. 2003; 442:284-93.
- Valsami S, et al. A clinicopathological study of B-cell differentiation markers and transcription factors in classical hodgkin lymphoma: a potential prognostic role of mum1/IRF4. Haematologica. 2007; 92:1343-50.
- Stein H, et al. Down regulation of Bob.1 /OBF.1 and oct2 in classical Hodgkins disease but no in lymphocyte perdominant Hodgkins disease correlates with immunglobulin transcription. Blood. 2001; 97:496-501.
- Hertel CB, et al. Loss of B cell identity correlates with loss of B Cell-specific transcription factors in Hodgkin / Reed-Sternberg Cells of classical Hodgkin lymphoma. Oncogene. 2002; 21:4908-20.
- Pileri SA, et al. Primary mediastinal B_cell lymphoma: high frequence of BCL-6 mutations and consistent expression of the transcription factors OCT-2, Bob.1 , PU.1 in the absence of immunoglobulins. Am J Pathology 2003; 162:243-53.
- Hoefnagel JJ, et al. Expression of B-cell transcription factors in primary cutaneous B-cell lymphoma. Moder Pathology. 2006; 19:1270-6.
- Greiner A, et al. Up-regulation of BOB.1/OBF.1 expression in normal germinal center B cells and germinal center-derived lymphomas. Am J Pathol. 2000;156:501-7.
- Kuroda H, et al. Primary diffuse large B-cell lymphoma of the breast. Breast Cancer. 2007; 14:317-22.
- Sáez AI, et al. Analysis of octamer-binding transcription factors Oct2 and Oct1 and their coactivator BOB.1/OBF.1 in lymphomas. Mod Pathol. 2002; 15:211-20.
- Loddenkemper C, et al. Differential Emu enhancer activity and expression of BOB.1/OBF.1, Oct2, PU.1, and immunoglobulin in reactive B-cell populations, B-cell non-Hodgkin lymphomas, and Hodgkin lymphomas. J Pathol. 2004; 202:60-9.
Specifications
- Reactivity: paraffin
- Visualization: cytoplasmic, nuclear
- Control: tonsil
- Dilution Range: 1:100-1:500*
Package Inserts
IFU
- IVD Rev. 3.0 (CMC29431030)
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Ordering Information
For in vitro diagnostic (IVD) use in USA
| 0.1 mL concentrate | 294R-14 |
| 0.5 mL concentrate | 294R-15 |
| 1 mL concentrate | 294R-16 |
| 1 mL predilute | 294R-17 |
| 7 mL predilute | 294R-18 |
For in vitro diagnostic (IVD) use in Canada
| 0.1 mL concentrate | 294R-14 |
| 0.5 mL concentrate | 294R-15 |
| 1 mL concentrate | 294R-16 |
| 1 mL predilute | 294R-17 |
| 7 mL predilute | 294R-18 |
For in vitro diagnostic (IVD) use in Europe
| 0.1 mL concentrate | 294R-14 |
| 0.5 mL concentrate | 294R-15 |
| 1 mL concentrate | 294R-16 |
| 1 mL predilute | 294R-17 |
| 7 mL predilute | 294R-18 |
For research use only (RUO) in Japan
| 0.1 mL concentrate | 294R-14-RUO |
| 0.5 mL concentrate | 294R-15-RUO |
| 1 mL concentrate | 294R-16-RUO |
| 1 mL predilute | 294R-17-RUO |
| 7 mL predilute | 294R-18-RUO |
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