Cell Marque Portfolio

Glypican-3 (1G12)

Mouse Monoclonal


Glypican-3 (GPC3) is a membrane-bound heparin sulfate proteoglycan known to participate in cell growth and differentiation.1 GPC3 expression has been observed in the majority of hepatocellular carcinomas (HCC), but is rarely expressed in non-neoplastic hepatic tissue, making it a useful marker for HCC.1-3 Additionally, this marker is expressed in many yolk sac tumors.1,4

Specialties: Gastrointestinal (GI) Pathology

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: hepatocellular carcinoma
  • Isotype: IgG1
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

References


  1. Kandil DH, et al. Glypican-3 a novel diagnostic marker for hepatocellular carcinoma and more. Adv Anat Pathol. 2009; 16:125-9.
  2. Coston WMP, et al. Distinction of hepatocellular carcinoma from benign hepatic mimickers using Glypican-3 and CD34 immunohistochemistry. Am J Surg Pathol. 2008; 32:433-44.
  3. Capurro M, et al. Glypican-3: a novel serum and histochemical marker for hepatocellular carcinoma. Gastroenterology. 2003; 125:89-97.
  4. Zynger DL, et al. Glypican 3: a novel marker in testicular germ cell tumors. Am J Surg Pathol. 2006; 30:1570-5.

Last updated: 2017-05-24 08:46:09

Glypican-3
Glypican-3 antibody displays a diffuse and cytoplasmic staining pattern in the neoplastic cells of hepatocellular carcinoma. Note non-neoplastic hepatocytes are negative for glypican-3.

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Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: hepatocellular carcinoma
  • Isotype: IgG1
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

References


  1. Kandil DH, et al. Glypican-3 a novel diagnostic marker for hepatocellular carcinoma and more. Adv Anat Pathol. 2009; 16:125-9.
  2. Coston WMP, et al. Distinction of hepatocellular carcinoma from benign hepatic mimickers using Glypican-3 and CD34 immunohistochemistry. Am J Surg Pathol. 2008; 32:433-44.
  3. Capurro M, et al. Glypican-3: a novel serum and histochemical marker for hepatocellular carcinoma. Gastroenterology. 2003; 125:89-97.
  4. Zynger DL, et al. Glypican 3: a novel marker in testicular germ cell tumors. Am J Surg Pathol. 2006; 30:1570-5.

Package Inserts

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Ordering Information

For in vitro diagnostic (IVD) use in USA

0.1 mL concentrate 261M-94
0.5 mL concentrate 261M-95
1 mL concentrate 261M-96
1 mL predilute 261M-97
7 mL predilute 261M-98
25 mL predilute 261M-90

For in vitro diagnostic (IVD) use in Canada

0.1 mL concentrate 261M-94
0.5 mL concentrate 261M-95
1 mL concentrate 261M-96
1 mL predilute 261M-97
7 mL predilute 261M-98
25 mL predilute 261M-90

For in vitro diagnostic (IVD) use in the EU

0.1 mL concentrate 261M-94
0.5 mL concentrate 261M-95
1 mL concentrate 261M-96
1 mL predilute 261M-97
7 mL predilute 261M-98
25 mL predilute 261M-90

For research use only (RUO) in Japan

0.1 mL concentrate 261M-94-RUO
0.5 mL concentrate 261M-95-RUO
1 mL concentrate 261M-96-RUO
1 mL predilute 261M-97-RUO
7 mL predilute 261M-98-RUO
25 mL predilute 261M-90-RUO

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: hepatocellular carcinoma
  • Isotype: IgG1
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

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References


  1. Kandil DH, et al. Glypican-3 a novel diagnostic marker for hepatocellular carcinoma and more. Adv Anat Pathol. 2009; 16:125-9.
  2. Coston WMP, et al. Distinction of hepatocellular carcinoma from benign hepatic mimickers using Glypican-3 and CD34 immunohistochemistry. Am J Surg Pathol. 2008; 32:433-44.
  3. Capurro M, et al. Glypican-3: a novel serum and histochemical marker for hepatocellular carcinoma. Gastroenterology. 2003; 125:89-97.
  4. Zynger DL, et al. Glypican 3: a novel marker in testicular germ cell tumors. Am J Surg Pathol. 2006; 30:1570-5.
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