Cell Marque Portfolio

Glypican-3 (1G12)

Mouse Monoclonal


Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Recently, GPC3 was identified to be a useful tumor marker for the diagnosis of HCC,1-3 hepatoblastoma,4 melanoma, testicular germ cell tumors, and Wilms’ tumor.5-6 In patients with HCC, GPC3 was over expressed in neoplastic liver tissue and elevated in serum, but was undetectable in normal liver, benign liver, and the serum of healthy donors.1 GPC3 expression was also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation.2 In the context of testicular germ cell tumors, GPC3 expression is up-regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma.5-6 A high level of GPC3 expression has also been found in some types of embryonal tumors, such as Wilms’ tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue.5 Together these studies indicate that GPC3 is an important tumor marker.

Specialties: Gastrointestinal (GI) Pathology

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: hepatocellular carcinoma
  • Isotype: IgG1
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

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References


  1. Capurro M, et al. Gastroenterology. 2003; 125:89-97.
  2. Coston WMP, et al. Am J Surg Pathol. 2008; 32:433-44.
  3. Kandil D, et al. Cancer. 2007; 111:316-22.
  4. Zynger DL, et al. Hum Pathol. 2008; 39:224-30.
  5. Kandil DH, et al. Adv Anat Pathol. 2009; 16:125-129.
  6. Zynger DL, et al. Am J Surg Pathol. 2006; 30:1570-1575.

Last updated: 2016-09-29 13:18:40

Glypican-3
Glypican-3 antibody displays a diffuse and cytoplasmic staining pattern in the neoplastic cells of hepatocellular carcinoma. Note non-neoplastic hepatocytes are negative for glypican-3.

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Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: hepatocellular carcinoma
  • Isotype: IgG1
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

References


  1. Capurro M, et al. Gastroenterology. 2003; 125:89-97.
  2. Coston WMP, et al. Am J Surg Pathol. 2008; 32:433-44.
  3. Kandil D, et al. Cancer. 2007; 111:316-22.
  4. Zynger DL, et al. Hum Pathol. 2008; 39:224-30.
  5. Kandil DH, et al. Adv Anat Pathol. 2009; 16:125-129.
  6. Zynger DL, et al. Am J Surg Pathol. 2006; 30:1570-1575.

Download an IFU

Select revision:



Ordering Information

For in vitro diagnostic (IVD) use in USA

DescriptionCat. No.
0.1 ml, concentrate 261M-94
0.5 ml, concentrate 261M-95
1 ml, concentrate 261M-96
1 ml, predilute 261M-97
7 ml, predilute 261M-98
25 ml, predilute 261M-90

For in vitro diagnostic (IVD) use in Canada

DescriptionCat. No.
0.1 ml, concentrate 261M-94
0.5 ml, concentrate 261M-95
1 ml, concentrate 261M-96
1 ml, predilute 261M-97
7 ml, predilute 261M-98
25 ml, predilute 261M-90

For in vitro diagnostic (IVD) use in the EU

DescriptionCat. No.
0.1 ml, concentrate 261M-94
0.5 ml, concentrate 261M-95
1 ml, concentrate 261M-96
1 ml, predilute 261M-97
7 ml, predilute 261M-98
25 ml, predilute 261M-90

For research use only (RUO) in Japan

DescriptionCat. No.
0.1 ml, concentrate 261M-94 (RUO)
0.5 ml, concentrate 261M-95 (RUO)
1 ml, concentrate 261M-96 (RUO)
1 ml, predilute 261M-97 (RUO)
7 ml, predilute 261M-98 (RUO)
25 ml, predilute 261M-90 (RUO)

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: hepatocellular carcinoma
  • Isotype: IgG1
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

Download an IFU

Select revision:


Keycode Specific IFU

Keycode:
Select prefix.
Please verify your keycode and try again. If problems presist contact Customer Service
Enter keycode.
Please verify your keycode and try again. If problems presist contact Customer Service

Some products may have special labeling requirements in the United States.

Are you located in the U.S.?


*Note: IFUs with the prefix RUO or ASR are only available for download in English.



Click here to learn about keycodes

References


  1. Capurro M, et al. Gastroenterology. 2003; 125:89-97.
  2. Coston WMP, et al. Am J Surg Pathol. 2008; 32:433-44.
  3. Kandil D, et al. Cancer. 2007; 111:316-22.
  4. Zynger DL, et al. Hum Pathol. 2008; 39:224-30.
  5. Kandil DH, et al. Adv Anat Pathol. 2009; 16:125-129.
  6. Zynger DL, et al. Am J Surg Pathol. 2006; 30:1570-1575.
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