ZytoVision® Probes for In Situ Hybridization
Complete FISH Workflow Solution
Precision Probes. Reliable Reagents. Implementation Kits. One Trusted Source. ZytoVision® offers a powerful portfolio of in situ hybridization (ISH) tools designed for the detection of genetic aberrations with clarity and confidence. Whether you're looking for high-performance, directly labeled probes or hands-free automation, the ZytoLight® and ZytoMation® product lines – distributed by Cell Marque Tissue Diagnostics – deliver streamlined solutions for laboratory workflows.
Key Highlights:
- Comprehensive portfolio of probes, reagents, and implementation kits
- High specificity and sensitivity for clear, confident results
- Flexible for laboratory workflows
ZytoLight® Products for FISH Analysis
ZytoLight® system uses direct-labeled FISH probes, which eliminate the need to detect the probes with fluorophore-coupled antibodies. ZytoLight® products are designed for the identification of genetic aberrations e.g. translocations, deletions, amplifications, and chromosomal aneuploidies by Fluorescence in situ Hybridization (FISH) in formalin-fixed, paraffin-embedded (FFPE) tissue sections or cytology specimens.
Key Benefits:
- Ready-to-use, directly labeled FISH probes
- High specificity and sensitivity for confident detection
- Suitable for both FFPE tissue and cytology specimens
- Visualize multiple gene states using innovative TriCheck™ design
Method Description & ZytoLight® Fluorochromes
The ZytoLight® system uses direct labeled FISH probes 1, eliminating the need to detect the probes with fluorophore-coupled antibodies. The probes are detected by fluorescence microscopy using appropriate filter sets 2. Due to an exciter filter 3, full-spectrum light, emitted by the microscope lamp 4, is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror 5. The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter 6 reduces the emitted light to a defined wavelength that can be detected.
ZytoLight® Kits – Convenient Solutions
For making FISH analysis reliable and user-friendly, ZytoLight® FISH probes are intended to be used with the appropriate Implementation Kit (annotated in the individual probe's instruction for use). The Implementation Kits include all necessary pretreatment solutions, wash buffers and DAPI/DuraTect-solution and a detailed protocol to perform successful FISH experiments.
ZytoMation® Products
The ZytoMation® probes combine the quality of the ZytoVision® probes for Fluorescence in situ Hybridization (FISH) with a streamlined workflow. They are designed to detect genetic aberrations such as translocations and amplifications in formalin-fixed, paraffin-embedded (FFPE) tissue sections.
Key Benefits:
- Streamlined 5-hour protocol
- Ready-to-use format for increased efficiency
- Minimized hands-on time with high reproducibility
Method Description
The ZytoMation® system uses direct-labeled FISH probes. The FISH signals are detected by fluorescence microscopy using appropriate filter sets.
For Research Use Only. Not for use in diagnostic procedures.