Nestin (10C2) Mouse Monoclonal Antibody

Nestin
Nestin antibody stains cytoplasmic for metastatic malignant melanoma.

Nestin (10C2) Mouse Monoclonal Antibody

Specialties: Dermatopathology

Updated: 2017-05-24 08:46:09

Nestin is a class VI intermediate filament (IF) protein. Nestin is expressed in neural progenitor cells during development of the central nervous system and peripheral nervous system. Cytoplasmic anti-nestin staining mostly occurs under pathological conditions. It has been reported that nestin expression is significantly increased in melanoma and correlated with more advanced stages of the disease.1 Nestin immunoreactivity is also reported in melanoma cells of HMB-45-negative amelanotic and melanotic, non-desmoplastic melanoma.2 Nestin is also useful in subclassifying breast carcinoma.4 Only cytoplasmic staining is considered positive, whereas any nuclear staining is considered as background artifact.

  1. Brychtova S, et al. Nestin expression in cutaneous melanomas and melanocytic nevi. J Cutan Pathol. 2007; 34:370-5.
  2. Kanoh M, et al. Nestin is expressed in HMB-45 negative melanoma cells in dermal parts of nodular melanoma. Journal of Dermatology. 2010; 37:505-11.
  3. Ishiwata T, et al. Nestin in gastrointestinal and other cancers: Effects on cells and tuor angiogenisis. World J Gastroenterol. 2011; 17:409-18.
  4. LI H, et al. Nestin is expressed in the basal/myoepithelial layer of the mammary gland and is a selective marker of basal epithelial breast tumors. Cancer Res. 2007; 67:501-10.

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: tonsil
  • Dilution Range: 1:25-1:100*

Package Inserts


IFU SDS

Ordering Information

For in vitro diagnostic (IVD) use in USA

0.1 mL concentrate388M-14
0.5 mL concentrate388M-15
1 mL concentrate388M-16
1 mL predilute388M-17
7 mL predilute388M-18

For in vitro diagnostic (IVD) use in Canada

0.1 mL concentrate388M-14
0.5 mL concentrate388M-15
1 mL concentrate388M-16
1 mL predilute388M-17
7 mL predilute388M-18

For in vitro diagnostic (IVD) use in Europe

0.1 mL concentrate388M-14
0.5 mL concentrate388M-15
1 mL concentrate388M-16
1 mL predilute388M-17
7 mL predilute388M-18

For research use only (RUO) in Japan

0.1 mL concentrate388M-14-RUO
0.5 mL concentrate388M-15-RUO
1 mL concentrate388M-16-RUO
1 mL predilute388M-17-RUO
7 mL predilute388M-18-RUO

 

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