Phosphohistone H3 (PHH3) Rabbit Polyclonal Antibody

Phosphohistone H3 (PHH3)
Mitotic figures labelled by the phosphohistone H3 antibody.

Phosphohistone H3 (PHH3) Rabbit Polyclonal Antibody

Specialties: Anatomic Pathology

Updated: 2018-01-24 14:38:22

Phosphohistone H3 (PHH3) is a core histone protein, which together with other histones, forms the major protein constituents of the chromatin in eukaryotic cells. In mammalian cells, phosphohistone H3 is negligible during interphase but reaches a maximum for chromatin condensation during mitosis.1 Immunohistochemical studies showed anti-PHH3 specifically detected the core protein histone H3 only when phosphorylated at serine 10 or serine 28. Studies have also revealed no phosphorylation on the histone H3 during apoptosis.2 PHH3 can serve as a mitotic marker to separate mitotic figures from apoptotic bodies and karyorrhectic debris, which may be a very useful tool in diagnosis of tumor grades, especially in CNS, skin, gyn., soft tissue, and GIST.3,4,5

  1. Gurley LR, et al. Histone phosphorylation and chromatin structure during mitosis in Chinese hamster cells.  Eur J Biochem 1978; 84:1-15.
  2. Hendzel MJ, et al. Chromatin condensation is not associated with apoptosis. J Biol Chem 1998; 273:24470-8.
  3. Colman H, et al. Assessment and prognostic significance of mitotic index using the mitosis marker phospho-histone H3 in low and intermediate-grade infiltrating astrocytomas. Am J Surg Pathol. 2006; 30:657-64.
  4. Nasr MR, et al. Comparison of pHH3, Ki-67, and survivin immunoreactivity in benign and malignant melanocytic lesions. Am J Dermatopathol. 2008; 30:117-22.
  5. Kim YJ, et al. Prognostic significance of the mitotic index using the mitosis marker anti-phosphohistone H3 in meningiomas Am J Clin Pathol. 2007; 128:118-25.

Specifications


  • Reactivity: paraffin
  • Control: Tonsil (Nuclear)
  • Dilution Range: 1:100-1:500 *

Package Inserts


IFU SDS

Ordering Information

For in vitro diagnostic (IVD) use in USA

25.0 mL predilute ready-to-use369A-10
0.1 mL concentrate369A-14
0.5 mL concentrate369A-15
1.0 mL concentrate369A-16
1.0 mL predilute ready-to-use369A-17
7.0 mL predilute ready-to-use369A-18

For in vitro diagnostic (IVD) use in Canada

25.0 mL predilute ready-to-use369A-10
0.1 mL concentrate369A-14
0.5 mL concentrate369A-15
1.0 mL concentrate369A-16
1.0 mL predilute ready-to-use369A-17
7.0 mL predilute ready-to-use369A-18

For in vitro diagnostic (IVD) use in Europe

25.0 mL predilute ready-to-use369A-10
0.1 mL concentrate369A-14
0.5 mL concentrate369A-15
1.0 mL concentrate369A-16
1.0 mL predilute ready-to-use369A-17
7.0 mL predilute ready-to-use369A-18

For research use only (RUO) in Japan

25.0 mL predilute ready-to-use (RUO)369A-10-RUO
0.1 mL concentrate (RUO)369A-14-RUO
0.5 mL concentrate (RUO)369A-15-RUO
1.0 mL concentrate (RUO)369A-16-RUO
1.0 mL predilute ready-to-use (RUO)369A-17-RUO
7.0 mL predilute ready-to-use (RUO)369A-18-RUO

 

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