Cell Marque Portfolio

Phosphohistone H3 (PHH3)

Rabbit Polyclonal


Phosphohistone H3 (PHH3) is a core histone protein, which together with other histones, forms the major protein constituents of the chromatin in eukaryotic cells. In mammalian cells, phosphohistone H3 is negligible during interphase but reaches a maximum for chromatin condensation during mitosis.1  Immunohistochemical studies showed anti-PHH3 specifically detected the core protein histone H3 only when phosphorylated at serine 10 or serine 28. Studies have also revealed no phosphorylation on the histone H3 during apoptosis.2  Therefore, PHH3 can serve as a mitotic marker to separate mitotic figures from apoptotic bodies and karyorrhectic debris, which may be a very useful tool in diagnosis of tumor grades, especially in CNS, skin, gyn., soft tissue, and GIST.3,4,5

Specialties: Anatomic Pathology

Specifications


  • Reactivity: paraffin
  • Visualization: nuclear
  • Control: tonsil
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

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References


  1. Gurley LR, et al. Eur J Biochem. 1978; 84:1-15.
  2. Hendzel MJ, et al. J Biol Chem. 1998; 273:24470-8.
  3. Colman H, et al. Am J Surg Pathol. 2006; 30:657-64.
  4. Nasr MR, et al. Am J Dermatopathol. 2008; 30:117-22.
  5. Kim YJ, et al. Am J Clin Pathol. 2007; 128:118-25.

Last updated: 2017-05-24 08:46:09

Phosphohistone H3 (PHH3)
Mitotic figures labelled by the phosphohistone H3 antibody.

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Specifications


  • Reactivity: paraffin
  • Visualization: nuclear
  • Control: tonsil
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

References


  1. Gurley LR, et al. Eur J Biochem. 1978; 84:1-15.
  2. Hendzel MJ, et al. J Biol Chem. 1998; 273:24470-8.
  3. Colman H, et al. Am J Surg Pathol. 2006; 30:657-64.
  4. Nasr MR, et al. Am J Dermatopathol. 2008; 30:117-22.
  5. Kim YJ, et al. Am J Clin Pathol. 2007; 128:118-25.

Download an IFU

Select revision:


Download SDS


Ordering Information

For in vitro diagnostic (IVD) use in USA

DescriptionCat. No.
0.1 ml, concentrate 369A-14
0.5 ml, concentrate 369A-15
1 ml, concentrate 369A-16
1 ml, predilute 369A-17
7 ml, predilute 369A-18
25 ml, predilute 369A-10

For in vitro diagnostic (IVD) use in Canada

DescriptionCat. No.
0.1 ml, concentrate 369A-14
0.5 ml, concentrate 369A-15
1 ml, concentrate 369A-16
1 ml, predilute 369A-17
7 ml, predilute 369A-18
25 ml, predilute 369A-10

For in vitro diagnostic (IVD) use in the EU

DescriptionCat. No.
0.1 ml, concentrate 369A-14
0.5 ml, concentrate 369A-15
1 ml, concentrate 369A-16
1 ml, predilute 369A-17
7 ml, predilute 369A-18
25 ml, predilute 369A-10

For research use only (RUO) in Japan

DescriptionCat. No.
0.1 ml, concentrate 369A-14-RUO
0.5 ml, concentrate 369A-15-RUO
1 ml, concentrate 369A-16-RUO
1 ml, predilute 369A-17-RUO
7 ml, predilute 369A-18-RUO
25 ml, predilute 369A-10-RUO

Specifications


  • Reactivity: paraffin
  • Visualization: nuclear
  • Control: tonsil
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

Download an IFU

Select revision:


Keycode Specific IFU

Keycode:
Select prefix.
Please verify your keycode and try again. If problems presist contact Customer Service
Enter keycode.
Please verify your keycode and try again. If problems presist contact Customer Service

Some products may have special labeling requirements in the United States.

Are you located in the U.S.?


*Note: IFUs with the prefix RUO or ASR are only available for download in English.



Click here to learn about keycodes

Download SDS

References


  1. Gurley LR, et al. Eur J Biochem. 1978; 84:1-15.
  2. Hendzel MJ, et al. J Biol Chem. 1998; 273:24470-8.
  3. Colman H, et al. Am J Surg Pathol. 2006; 30:657-64.
  4. Nasr MR, et al. Am J Dermatopathol. 2008; 30:117-22.
  5. Kim YJ, et al. Am J Clin Pathol. 2007; 128:118-25.
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