Cytokeratin (CAM 5.2) Mouse Monoclonal Antibody

Cytokeratin
Cytokeratin (CAM 5.2) on breast.

Cytokeratin (CAM 5.2) Mouse Monoclonal Antibody

Specialties: Anatomic Pathology

Updated: 2018-01-11 11:14:43

Anti-CAM 5.2 is a mouse monoclonal antibody that was generated by using the human colorectal carcinoma cell line HT24. Anti-CAM 5.2 is not technically a pan-keratin antibody because its reactivity has been reported to be restricted primarily to a few keratins. However, since the few keratins are widely expressed in formalin-fixed, paraffin-embedded tissues, especially in simple and glandular epithelia, this antibody can be successfully used as a reliable marker for neoplasms of epithelial differentiation. Anti-CAM 5.2 reacts with the majority of epithelial tumors, including lung, liver, pancreas, GI tract, breast, genitourinary system, female reproductive organs and some endocrine organs.1  Adrenal cortical carcinomas frequently do not react with anti-CAM 5.2.2 Sebaceous carcinoma, squamous cell carcinoma, and basal cell carcinoma may show an overlapping morphology. Anti-CAM 5.2 is useful to discriminate among them. Literature indicates no reactivity by anti-CAM 5.2 for squamous cell carcinoma while sebaceous carcinoma and basal cell carcinoma show 73% and 44% immunoreactivity respectively.3 Similar to other broad-spectrum keratin antibodies, such as the AE1/AE3 antibody cocktail, anti-CAM 5.2 may stain astrocytic tumors, but the positivity reported for this tumor cross-reactivity is lower than with anti-AE1/AE3.4
 

  1. Ordóñez NG. Broad-spectrum immunohistochemical epithelial markers: a review. Hum Pathol. 2013; 44:1195-215.
  2. Pan CC, et al.  Differential immunoprofiles of hepatocellular carcinoma, renal cell carcinoma, and adrenocortical carcinoma: a systemic immunohistochemical survey using tissue array technique. Appl Immunohistochem Mol Morphol. 2005; 13:347-52.
  3. Sinard JH. Immunohistochemical distinction of ocular sebaceous carcinoma from basal cell and squamous cell carcinoma. Arch Ophthalmol. 1999; 117:776–83..
  4. Cosgrove MM, et al. Keratin intermediate filament expression in astrocytic neoplasms: analysis by immunocytochemistry, western blot, and northern hybridization. Mod Pathol. 1993; 6:342-7.

Specifications


  • Reactivity: paraffin
  • Control: Hepatocellular carcinoma (Cytoplasmic); Appendix (Cytoplasmic)
  • Dilution Range: 1:25-1:100 *

Package Inserts


IFU SDS

Ordering Information

For in vitro diagnostic (IVD) use in USA

25.0 mL predilute ready-to-use452M-90
0.1 mL concentrate452M-94
0.5 mL concentrate452M-95
1.0 mL concentrate452M-96
1.0 mL predilute ready-to-use452M-97
7.0 mL predilute ready-to-use452M-98

For in vitro diagnostic (IVD) use in Canada

25.0 mL predilute ready-to-use452M-90
0.1 mL concentrate452M-94
0.5 mL concentrate452M-95
1.0 mL concentrate452M-96
1.0 mL predilute ready-to-use452M-97
7.0 mL predilute ready-to-use452M-98

For in vitro diagnostic (IVD) use in Europe

25.0 mL predilute ready-to-use452M-90
0.1 mL concentrate452M-94
0.5 mL concentrate452M-95
1.0 mL concentrate452M-96
1.0 mL predilute ready-to-use452M-97
7.0 mL predilute ready-to-use452M-98

For research use only (RUO) in Japan

25.0 mL predilute ready-to-use (RUO)452M-90-RUO
0.1 mL concentrate (RUO)452M-94-RUO
0.5 mL concentrate (RUO)452M-95-RUO
1.0 mL concentrate (RUO)452M-96-RUO
1.0 mL predilute ready-to-use (RUO)452M-97-RUO
7.0 mL predilute ready-to-use (RUO)452M-98-RUO

 

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