Cell Marque Portfolio

Cytokeratin (CAM 5.2)

Mouse Monoclonal


Anti-CAM 5.2 is a mouse monoclonal antibody that was generated by using the human colorectal carcinoma cell line HT24. Anti-CAM 5.2 is not technically a pan-keratin antibody because its reactivity has been reported to be restricted primarily to a few keratins. However, since the few keratins are widely expressed in formalin-fixed, paraffin-embedded tissues, especially in simple and glandular epithelia, this antibody can be successfully used as a reliable marker for neoplasms of epithelial differentiation. Anti-CAM 5.2 reacts with the majority of epithelial tumors, including lung, liver, pancreas, GI tract, breast, genitourinary system, female reproductive organs and some endocrine organs.Hepatocellular carcinoma is targeted by anti-CAM 5.2, but not by pan-cytokeratin AE1/AE3 antibody.1 Adrenal cortical carcinomas frequently do not react with anti-CAM 5.2.Sebaceous carcinoma, squamous cell carcinoma, and basal cell carcinoma may show an overlapping morphology. Anti-CAM 5.2 is useful to discriminate among them. Literature indicates no reactivity by anti-CAM 5.2 for squamous cell carcinoma while sebaceous carcinoma and basal cell carcinoma show 73% and 44% immunoreactivity respectively.Similar to other broad-spectrum keratin antibodies, such as the AE1/AE3 antibody cocktail, anti-CAM 5.2 may stain astrocytic tumors, but the positivity reported for this tumor cross-reactivity is lower than with anti-AE1/AE3.4

Specialties: Anatomic Pathology

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: appendix, hepatocellular carcinoma
  • Isotype: IgG2a/k
  • Dilution Range: 1:25-1:100*

Protocols


  • Cell Marque HiDef
    Protease/30-60minAb/HiDef/Perm Red
  • Cell Marque HiDef
    Protease/30-60minAb/HiDef/DAB
  • Ventana OptiView
    OV/P1(4min)/8min @ 37°C
  • Ventana ultraView
    UV/P1(4min)/16min @ 37°C

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Literature

Cytokeratin
Cytokeratin (CAM 5.2) on breast.

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Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: appendix, hepatocellular carcinoma
  • Isotype: IgG2a/k
  • Dilution Range: 1:25-1:100*

Protocols


  • Cell Marque HiDef
    Protease/30-60minAb/HiDef/Perm Red
  • Cell Marque HiDef
    Protease/30-60minAb/HiDef/DAB
  • Ventana OptiView
    OV/P1(4min)/8min @ 37°C
  • Ventana ultraView
    UV/P1(4min)/16min @ 37°C

Download an IFU

Select revision:



Ordering Information

For in vitro diagnostic (IVD) use in USA

DescriptionCat. No.
0.1 ml, concentrate 452M-94
0.5 ml, concentrate 452M-95
1 ml, concentrate 452M-96
1 ml, predilute 452M-97
7 ml, predilute 452M-98
25 ml, predilute 452M-90

For in vitro diagnostic (IVD) use in Canada

DescriptionCat. No.
0.1 ml, concentrate 452M-94
0.5 ml, concentrate 452M-95
1 ml, concentrate 452M-96
1 ml, predilute 452M-97
7 ml, predilute 452M-98
25 ml, predilute 452M-90

For in vitro diagnostic (IVD) use in the EU

DescriptionCat. No.
0.1 ml, concentrate 452M-94
0.5 ml, concentrate 452M-95
1 ml, concentrate 452M-96
1 ml, predilute 452M-97
7 ml, predilute 452M-98
25 ml, predilute 452M-90

For research use only (RUO) in Japan

DescriptionCat. No.
0.1 ml, concentrate 452M-94 (RUO)
0.5 ml, concentrate 452M-95 (RUO)
1 ml, concentrate 452M-96 (RUO)
1 ml, predilute 452M-97 (RUO)
7 ml, predilute 452M-98 (RUO)
25 ml, predilute 452M-90 (RUO)

Literature

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: appendix, hepatocellular carcinoma
  • Isotype: IgG2a/k
  • Dilution Range: 1:25-1:100*

Protocols


  • Cell Marque HiDef
    Protease/30-60minAb/HiDef/Perm Red
  • Cell Marque HiDef
    Protease/30-60minAb/HiDef/DAB
  • Ventana OptiView
    OV/P1(4min)/8min @ 37°C
  • Ventana ultraView
    UV/P1(4min)/16min @ 37°C

Download an IFU

Select revision:


Keycode Specific IFU

Keycode:
Select prefix.
Please verify your keycode and try again. If problems presist contact Customer Service
Enter keycode.
Please verify your keycode and try again. If problems presist contact Customer Service

Some products may have special labeling requirements in the United States.

Are you located in the U.S.?


*Note: IFUs with the prefix RUO or ASR are only available for download in English.



Click here to learn about keycodes

Request More Information