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CD117, c-kit (YR145)

Rabbit Monoclonal


CD117, c-kit is a tyrosine kinase receptor found on interstitial cells of Cajal, germ cells, bone marrow cells, melanocytes, and mast cells. CD117 expression by immunohistochemistry is considered a key diagnostic feature in gastrointestinal stromal tumors (GISTs) and is seen in most of these tumors. The staining pattern of CD117 expression in GISTs is typically strong and diffuse, showing a pancytoplasmic and sometimes membranous pattern. In addition, some GISTs, especially extraintestinal or epithelioid GISTs show a cytoplasmic ‘‘dotlike’’ immunostaining pattern.1 When used in combination with other markers, the sensitivity and specificity of CD117 antibody increases. The panel, including CD117, CD34, S-100, desmin, and SMA, can effectively differentiate between GISTs, true smooth-muscle tumors, and neural tumors, because GISTs do not typically express desmin or S100, but demonstrate immunopositivity for CD117 and/or CD34 and occasionally SMA. Therefore, within the spectrum of mesenchymal neoplasms of the GI tract, CD117, CD34, desmin, S-100, and SMA should be the primary panel of the choice for most circumstances.2 A common, morphologically, and clinically important differential diagnosis of germ cell tumors is the distinction of seminoma from embryonal carcinoma. A 2-antibody panel, CD117 and CD30 with opposite patterns of reactivity, is recommended and CD117 can be demonstrated in 95% to 100% of seminoma as well as intratubular germ cell neoplasia, but is negative in almost all embryonal carcinoma.3 The morphologic distinction of clear cell renal cell carcinoma (RCC), chromophobe RCC, eosinophilic and oncocytoma sometimes generates difficulties, especially for high grade tumors. A panel of three antibodies, including CD117, CK7 and CA IX, would help to separate them. Clear cell RCC will display diffuse membranous staining of the tumor cells by CA IX, but is negative for CD117 and CK7; chromophobe RCC will be positive for CK7 (cytoplasmic) and CD117 (membranous); whereas CD117 highlights oncocytoma in the membrane, but is almost completely negative for CK7.4 Disorders of mast cells, although uncommon, can be difficult to diagnose because of morphologic overlap with other diseases such as B-cell and T-cell lymphomas, acute and chronic myelogenous leukemia, myelodysplastic syndromes, and histocytic proliferations. Mast cells are well known to show distinctive membranous and cytoplasmic staining for CD117. Furthermore, similar staining is seen in mast cell disorders and can be used to identify neoplasms of mast cells in bone marrow, skin, lymph nodes, and solid organs.5

Specialties: Anatomic Pathology

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic, membranous
  • Control: gastrointestinal stromal tumor, seminoma
  • Isotype: IgG
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

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References


  1. Turner MS, et al. Arch Pathol Lab Med. 2009; 133:1370-4.
  2. Gibson PC, et al. Adv Anat Pathol. 2002; 9:65-9.
  3. Ulbright TM, et al. Am J Surg Pathol. 2014; 38:e50-9.
  4. Reuter VE, et al. Am J Surg Pathol. 2014; 38:e35-49.
  5. Natkunam Y, et al. Am J Surg Pathol. 2000; 24:81-91.

Last updated: 2017-02-21 15:26:20

CD117, c-kit
CD117, c-kit (YR145) on stomach.

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Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic, membranous
  • Control: gastrointestinal stromal tumor, seminoma
  • Isotype: IgG
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

References


  1. Turner MS, et al. Arch Pathol Lab Med. 2009; 133:1370-4.
  2. Gibson PC, et al. Adv Anat Pathol. 2002; 9:65-9.
  3. Ulbright TM, et al. Am J Surg Pathol. 2014; 38:e50-9.
  4. Reuter VE, et al. Am J Surg Pathol. 2014; 38:e35-49.
  5. Natkunam Y, et al. Am J Surg Pathol. 2000; 24:81-91.

Download an IFU

No IFUs found.

Ordering Information

For in vitro diagnostic (IVD) use in Canada

DescriptionCat. No.
0.1 ml, concentrate 117R-14
0.5 ml, concentrate 117R-15
1 ml, concentrate 117R-16
1 ml, predilute 117R-17
7 ml, predilute 117R-18
25 ml, predilute 117R-10

For in vitro diagnostic (IVD) use in the EU

DescriptionCat. No.
0.1 ml, concentrate 117R-14
0.5 ml, concentrate 117R-15
1 ml, concentrate 117R-16
1 ml, predilute 117R-17
7 ml, predilute 117R-18
25 ml, predilute 117R-10

For research use only (RUO) in Japan

DescriptionCat. No.
0.1 ml, concentrate 117R-14 (RUO)
0.5 ml, concentrate 117R-15 (RUO)
1 ml, concentrate 117R-16 (RUO)
1 ml, predilute 117R-17 (RUO)
7 ml, predilute 117R-18 (RUO)
25 ml, predilute 117R-10 (RUO)

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic, membranous
  • Control: gastrointestinal stromal tumor, seminoma
  • Isotype: IgG
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

Download an IFU

Select revision:


No IFUs found.

Keycode Specific IFU

Keycode:
Select prefix.
Please verify your keycode and try again. If problems presist contact Customer Service
Enter keycode.
Please verify your keycode and try again. If problems presist contact Customer Service

Some products may have special labeling requirements in the United States.

Are you located in the U.S.?


*Note: IFUs with the prefix RUO or ASR are only available for download in English.



Click here to learn about keycodes

References


  1. Turner MS, et al. Arch Pathol Lab Med. 2009; 133:1370-4.
  2. Gibson PC, et al. Adv Anat Pathol. 2002; 9:65-9.
  3. Ulbright TM, et al. Am J Surg Pathol. 2014; 38:e50-9.
  4. Reuter VE, et al. Am J Surg Pathol. 2014; 38:e35-49.
  5. Natkunam Y, et al. Am J Surg Pathol. 2000; 24:81-91.
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