T-bet (MRQ-46) Rabbit Monoclonal Antibody
Specialties: Hematopathology
T-bet, a T-box transcription factor, is expressed in CD4+ T-lymphocytes committed to T-helper (Th)1 T-cell development from naïve T-helper precursor cells (Thp) and redirects Th2 T-cells to Th1 development. Anti-T-bet is a marker of mature T-cells and is expressed at very low levels in Thp cells and is absent in precursor T-lymphoblastic leukemia/lymphoma cells. Scattered small lymphocytes in the interfollicular T-cell zone of reactive lymphoid tissue, including tonsil, lymph node, and spleen exhibited nuclear staining for anti-T-bet, with no anti-T-bet staining observed in germinal centers or mantle or marginal zones. T-bet is expressed in a significant subset of B-cell lymphoproliferative disorders, particularly at an early stage of B-cell development (precursor B-cell lymphoblastic leukemia/lymphoblastic lymphoma), and B-cell neoplasms derived from mature B-cells, including CLL/SLL, marginal zone lymphoma, and hairy cell leukemia. In contrast, B-cell neoplasms derived from pre-germinal center or germinal center B-cells, including mantle cell lymphoma, follicular lymphoma, diffuse large B-cell lymphoma, and Burkitt lymphoma are negative for T-bet.1-7
- Szabo SJ, et al. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell. 2000; 100:665-69.
- Zhang WX, et al. Cloning and characterization of a new member of the T-box gene family. Genomics. 2000; 70:41-8.
- Jöhrens K, et al. T-bet transcription factor detection facilitates the diagnosis of minimal hairy cell leukemia infiltrates in bone marrow trephines. Am J Surg Pathol. 2007; 31:1181-5.
- Atayar C, et al. Expression of the T-cell transcription factors, GATA-3 and T-bet, in the neoplastic cells of Hodgkin lymphomas. Am J Pathol. 2005; 166:127-34.
- Dorfman DM, et al. T-bet, a T-cell associated transcription factor, is expressed in a subset of B-cell lymphoproliferative disorders. Am J Clin Pathol. 2004; 122:292-7.
- Harashima A, et al. Transcription factor expression in B-cell precursor-leukemia cell lines: preferential expression of T-bet. Leuk Res. 2005; 29:841-8.
- Marafioti T, et al. Expression of B-cell lymphocyte-associated transcription factors in human T-cell neoplasms. Am J Pathol. 2003; 162:861-71.
Specifications
- Reactivity: paraffin
- Control: Tonsil (Nuclear); Hairy Cell Leukemia (Nuclear)
- Dilution Range: 1:100-1:500 *
Package Inserts
IFU
- IVD Rev. 4.0 (CMC36837040)
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Ordering Information
For in vitro diagnostic (IVD) use in USA
| 0.1 mL concentrate | 368R-74 |
| 0.5 mL concentrate | 368R-75 |
| 1.0 mL concentrate | 368R-76 |
| 1.0 mL predilute ready-to-use | 368R-77 |
| 7.0 mL predilute ready-to-use | 368R-78 |
For in vitro diagnostic (IVD) use in Canada
| 0.1 mL concentrate | 368R-74 |
| 0.5 mL concentrate | 368R-75 |
| 1.0 mL concentrate | 368R-76 |
| 1.0 mL predilute ready-to-use | 368R-77 |
| 7.0 mL predilute ready-to-use | 368R-78 |
For in vitro diagnostic (IVD) use in Europe
| 0.1 mL concentrate | 368R-74 |
| 0.5 mL concentrate | 368R-75 |
| 1.0 mL concentrate | 368R-76 |
| 1.0 mL predilute ready-to-use | 368R-77 |
| 7.0 mL predilute ready-to-use | 368R-78 |
For research use only (RUO) in Japan
| 0.1 mL concentrate (RUO) | 368R-74-RUO |
| 0.5 mL concentrate (RUO) | 368R-75-RUO |
| 1.0 mL concentrate (RUO) | 368R-76-RUO |
| 1.0 mL predilute ready-to-use (RUO) | 368R-77-RUO |
| 7.0 mL predilute ready-to-use (RUO) | 368R-78-RUO |
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