Cell Marque Portfolio

CD3

Rabbit Polyclonal


CD3’s immunohistochemical detection locates the cytoplasmic component of CD3 protein. Anti-CD3 is considered to be a pan-T-cell marker and reacts with an antigen present at the surface and in the cytoplasm of T lymphocytes. Anti-CD3 is widely used for the identification of immature and mature T-cell malignancies.1-8

Specialties: Hematopathology

Specifications


  • Reactivity: paraffin
  • Visualization: membranous
  • Control: tonsil
  • Dilution Range: 1:100-1:1000*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C

References


  1. Denning SM, et al. Activation of human thymocytes via CD3 and CD2 molecules. In: McMichael AJ, et al. eds. Leucocyte Typing III, White Cell Differentiation Antigens. Oxford-New York-Tokyo. Oxford University Press. 1987; 144-7.
  2. Beverley PC, et al. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981; 11:329-34.
  3. Clevers H, et al. The transmembrane orientation of the epsilon chain of the TcR/CD3 complex. Eur J Immunol. 1988; 18:705-10.
  4. Campana D, et al. The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. J Immunol. 1987; 138:648-55.
  5. Hedvat CV, et al. Application of tissue microarray technology to the study of non-Hodgkin's and Hodgkin's lymphoma. Hum Pathol. 2002; 33:968-74.
  6. Karube K, et al. Non-B, non-T neoplasms with lymphoblast morphology: further clarification and classification. Am J Surg Pathol. 2003; 27:1366-74.
  7. Dogan A, et al. Micronodular T-cell/histiocyte-rich large B-cell lymphoma of the spleen: histology, immunophenotype, and differential diagnosis. Am J Surg Pathol. 2003; 27:903-11.
  8. Axdorph U, et al. T-cell-rich B-cell lymphoma - diagnostic and therapeutic aspects. APMIS. 2002; 110:379-90.

Last updated: 2017-05-24 08:46:09

CD3
CD3 polyclonal antibody stains interfollicular T-cells in a cytoplasmic staining pattern.

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Specifications


  • Reactivity: paraffin
  • Visualization: membranous
  • Control: tonsil
  • Dilution Range: 1:100-1:1000*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C

References


  1. Denning SM, et al. Activation of human thymocytes via CD3 and CD2 molecules. In: McMichael AJ, et al. eds. Leucocyte Typing III, White Cell Differentiation Antigens. Oxford-New York-Tokyo. Oxford University Press. 1987; 144-7.
  2. Beverley PC, et al. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981; 11:329-34.
  3. Clevers H, et al. The transmembrane orientation of the epsilon chain of the TcR/CD3 complex. Eur J Immunol. 1988; 18:705-10.
  4. Campana D, et al. The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. J Immunol. 1987; 138:648-55.
  5. Hedvat CV, et al. Application of tissue microarray technology to the study of non-Hodgkin's and Hodgkin's lymphoma. Hum Pathol. 2002; 33:968-74.
  6. Karube K, et al. Non-B, non-T neoplasms with lymphoblast morphology: further clarification and classification. Am J Surg Pathol. 2003; 27:1366-74.
  7. Dogan A, et al. Micronodular T-cell/histiocyte-rich large B-cell lymphoma of the spleen: histology, immunophenotype, and differential diagnosis. Am J Surg Pathol. 2003; 27:903-11.
  8. Axdorph U, et al. T-cell-rich B-cell lymphoma - diagnostic and therapeutic aspects. APMIS. 2002; 110:379-90.

Package Inserts

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Ordering Information

For in vitro diagnostic (IVD) use in USA

0.1 mL concentrate 103A-74
0.5 mL concentrate 103A-75
1 mL concentrate 103A-76
1 mL predilute 103A-77
7 mL predilute 103A-78
25 mL predilute 103A-70

For in vitro diagnostic (IVD) use in Canada

0.1 mL concentrate 103A-74
0.5 mL concentrate 103A-75
1 mL concentrate 103A-76
1 mL predilute 103A-77
7 mL predilute 103A-78
25 mL predilute 103A-70

For in vitro diagnostic (IVD) use in the EU

0.1 mL concentrate 103A-74
0.5 mL concentrate 103A-75
1 mL concentrate 103A-76
1 mL predilute 103A-77
7 mL predilute 103A-78
25 mL predilute 103A-70

For research use only (RUO) in Japan

0.1 mL concentrate 103A-74-RUO
0.5 mL concentrate 103A-75-RUO
1 mL concentrate 103A-76-RUO
1 mL predilute 103A-77-RUO
7 mL predilute 103A-78-RUO
25 mL predilute 103A-70-RUO

Specifications


  • Reactivity: paraffin
  • Visualization: membranous
  • Control: tonsil
  • Dilution Range: 1:100-1:1000*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1M/16min @ 37°C

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References


  1. Denning SM, et al. Activation of human thymocytes via CD3 and CD2 molecules. In: McMichael AJ, et al. eds. Leucocyte Typing III, White Cell Differentiation Antigens. Oxford-New York-Tokyo. Oxford University Press. 1987; 144-7.
  2. Beverley PC, et al. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981; 11:329-34.
  3. Clevers H, et al. The transmembrane orientation of the epsilon chain of the TcR/CD3 complex. Eur J Immunol. 1988; 18:705-10.
  4. Campana D, et al. The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. J Immunol. 1987; 138:648-55.
  5. Hedvat CV, et al. Application of tissue microarray technology to the study of non-Hodgkin's and Hodgkin's lymphoma. Hum Pathol. 2002; 33:968-74.
  6. Karube K, et al. Non-B, non-T neoplasms with lymphoblast morphology: further clarification and classification. Am J Surg Pathol. 2003; 27:1366-74.
  7. Dogan A, et al. Micronodular T-cell/histiocyte-rich large B-cell lymphoma of the spleen: histology, immunophenotype, and differential diagnosis. Am J Surg Pathol. 2003; 27:903-11.
  8. Axdorph U, et al. T-cell-rich B-cell lymphoma - diagnostic and therapeutic aspects. APMIS. 2002; 110:379-90.
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