MCM3 (EP202) Rabbit Monoclonal Antibody

MCM3
Nuclear staining by anti-MCM3 on cervical squamous cell carcinoma.

MCM3 (EP202) Rabbit Monoclonal Antibody

Specialties: Head/Neck Pathology

Updated: 2017-05-24 08:46:09
MCM3, minichromosome maintenance protein 3, belongs to the MCM family of highly conserved group of DNA-binding proteins known to have a role in the initiation and regulation of DNA replication during the cell cycle.1-7 MCM3 expression is up-regulated in proliferating cells and absent in terminally differentiated cells. Like Ki-67, MCM3 marks proliferating cells. MCM3 stains proliferating cells like Ki-67, and in addition marks cells present in the intermediate layer of epithelium (which are typically negative for Ki-67). Both Ki-67 and MCM3 are not detectable in differentiated cells.6 However, unlike Ki-67, MCM3 protein down-regulation is delayed. This suggests that Ki-67 may be expressed during a shorter interval of the cell cycle than MCM3. Anti-MCM3 may be a more reliable proliferation marker than anti-Ki-67.1
  1. Lee YS, et al. Minichromosome maintenance protein 3 is a candidate proliferation marker in papillary thyroid carcinoma. Experimental and Molecular Pathology. 2010; 88:138-42.
  2. Gambichler T, et al. Minichromosome maintenance proteins are useful adjuncts to differentiate between benign and malignant melanocytic skin lesions. J Am Acad Dermatol. 2009; 60:808-13.
  3. Musahl C, et al. Stability of the replicative Mcm3 protein in proliferating and differentiating human cells. Experimental Cell Research. 1998; 241:260-64.
  4. Das M, et al. MCM paradox: abundance of eukaryotic replicative helicases and genomic integrity. Hindawi Publishing Corporation. 2014; 10:1-11.
  5. Tye BK, et al. The hexameric eukaryotic MCM helicase: building symmetry from nonidentical parts. The Journal of Biological Chemistry. 2000; 275:34833-6.
  6. Endl E, et al. The expression of Ki-67, MCM3, and p27 defines distinct subsets of proliferating, resting, and differentiated cells. Journal of Pathology. 2001; 195:457-62.
  7. Lei M, et al. Initiating DNA synthesis: from recruiting to activating the MCM complex. Journal of Cell Science. 2001; 114:1447-54.

Specifications


  • Reactivity: paraffin
  • Visualization: nuclear
  • Control: tonsil
  • Dilution Range: 1:25-1:100*

Package Inserts


IFU SDS

Ordering Information

For in vitro diagnostic (IVD) use in USA

0.1 mL concentrate435R-14
0.5 mL concentrate435R-15
1 mL concentrate435R-16
1 mL predilute435R-17
7 mL predilute435R-18

For in vitro diagnostic (IVD) use in Canada

0.1 mL concentrate435R-14
0.5 mL concentrate435R-15
1 mL concentrate435R-16
1 mL predilute435R-17
7 mL predilute435R-18

For in vitro diagnostic (IVD) use in Europe

0.1 mL concentrate435R-14
0.5 mL concentrate435R-15
1 mL concentrate435R-16
1 mL predilute435R-17
7 mL predilute435R-18

For research use only (RUO) in Japan

0.1 mL concentrate435R-14-RUO
0.5 mL concentrate435R-15-RUO
1 mL concentrate435R-16-RUO
1 mL predilute435R-17-RUO
7 mL predilute435R-18-RUO

 

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