Cell Marque Portfolio

Actin, Smooth Muscle (1A4)

Mouse Monoclonal


Smooth Muscle Actin is a part of the actin family of proteins which are highly conserved and form microfilaments. These filaments are one of the major components of the cytoskeleton. Anti-smooth muscle actin immunohistochemical reactivity is seen in smooth muscle cells, myofibroblasts and myoepithelial cells.1-3

Specialties: Anatomic Pathology Soft Tissue Pathology

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: appendix, uterus, vessel wall
  • Isotype: IgG/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/32min @ RT + AMP

References


  1. Cooke PH. A filamentous cytoskeleton in vertebrate smooth muscle fibers. J Cell Biol. 1976; 68:539-56.
  2. Skalli O, et al. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol. 1986; 103:2787-96.
  3. Perez-Montiel MD, et al. Differential expression of smooth muscle myosin, smooth muscle actin, h-caldesmon, and calponin in the diagnosis of myofibroblastic and smooth muscle lesions of skin and soft tissue. Am J Dermatopathol. 2006; 28:105-11.

Last updated: 2017-05-24 08:46:09

Actin, Smooth Muscle
Smooth muscle actin antibody detects actin expression in the cytoplasm.

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Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: appendix, uterus, vessel wall
  • Isotype: IgG/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/32min @ RT + AMP

References


  1. Cooke PH. A filamentous cytoskeleton in vertebrate smooth muscle fibers. J Cell Biol. 1976; 68:539-56.
  2. Skalli O, et al. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol. 1986; 103:2787-96.
  3. Perez-Montiel MD, et al. Differential expression of smooth muscle myosin, smooth muscle actin, h-caldesmon, and calponin in the diagnosis of myofibroblastic and smooth muscle lesions of skin and soft tissue. Am J Dermatopathol. 2006; 28:105-11.

Package Inserts

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Ordering Information

For in vitro diagnostic (IVD) use in USA

DescriptionCat. No.
0.1 mL concentrate 202M-94
0.5 mL concentrate 202M-95
1 mL concentrate 202M-96
1 mL predilute 202M-97
7 mL predilute 202M-98
25 mL predilute 202M-90

For in vitro diagnostic (IVD) use in Canada

DescriptionCat. No.
0.1 mL concentrate 202M-94
0.5 mL concentrate 202M-95
1 mL concentrate 202M-96
1 mL predilute 202M-97
7 mL predilute 202M-98
25 mL predilute 202M-90

For in vitro diagnostic (IVD) use in the EU

DescriptionCat. No.
0.1 mL concentrate 202M-94
0.5 mL concentrate 202M-95
1 mL concentrate 202M-96
1 mL predilute 202M-97
7 mL predilute 202M-98
25 mL predilute 202M-90

For research use only (RUO) in Japan

DescriptionCat. No.
0.1 mL concentrate 202M-94-RUO
0.5 mL concentrate 202M-95-RUO
1 mL concentrate 202M-96-RUO
1 mL predilute 202M-97-RUO
7 mL predilute 202M-98-RUO
25 mL predilute 202M-90-RUO

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: appendix, uterus, vessel wall
  • Isotype: IgG/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/32min @ RT + AMP

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References


  1. Cooke PH. A filamentous cytoskeleton in vertebrate smooth muscle fibers. J Cell Biol. 1976; 68:539-56.
  2. Skalli O, et al. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol. 1986; 103:2787-96.
  3. Perez-Montiel MD, et al. Differential expression of smooth muscle myosin, smooth muscle actin, h-caldesmon, and calponin in the diagnosis of myofibroblastic and smooth muscle lesions of skin and soft tissue. Am J Dermatopathol. 2006; 28:105-11.
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