Cell Marque Portfolio

Actin, Smooth Muscle (1A4)

Mouse Monoclonal


Actin is a major component of the cytoskeleton and is present in every cell type. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-Smooth Muscle Actin antibody does not stain cardiac or skeletal muscle; however, it will stain myofibroblasts and myoepithelial cells. This antibody could be used together with Muscle Specific Actin to distinguish leiomyosarcoma from rhabdomyosarcoma. In most cases of rhabdomyosarcoma, this antibody gives negative results whereas Muscle Specific Actin is positive in the rhabdomyoblasts. Leiomyosarcomas are positive with both Muscle Specific Actin and Smooth Muscle Actin antibodies. 1-5

Specialties: Anatomic Pathology Soft Tissue Pathology

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: appendix, uterus, vessel wall
  • Isotype: IgG/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/32min @ RT + AMP

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Keycode Specific IFU

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*Note: IFUs with the prefix RUO or ASR are only available for download in English.


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References


  1. Cooke PH. J Cell Biol. 1976; 68:539-556.
  2. Skalli O, et al. J Cell Biol. 1986; 103:2787-2796.
  3. Gown AM, et al. J Cell Biol. 1985; 100:807- 813.
  4. Kuroda M. Biochem Biophys Acta. 1985; 843:20-213.
  5. Lazarides E. J Histochem Cytochem. 1975; 223:507-528.

Last updated: 2016-01-07 14:13:45

Actin, Smooth Muscle
Smooth muscle actin antibody detects actin expression in the cytoplasm.

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Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: appendix, uterus, vessel wall
  • Isotype: IgG/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/32min @ RT + AMP

References


  1. Cooke PH. J Cell Biol. 1976; 68:539-556.
  2. Skalli O, et al. J Cell Biol. 1986; 103:2787-2796.
  3. Gown AM, et al. J Cell Biol. 1985; 100:807- 813.
  4. Kuroda M. Biochem Biophys Acta. 1985; 843:20-213.
  5. Lazarides E. J Histochem Cytochem. 1975; 223:507-528.

Download an IFU

Select revision:



Ordering Information

For in vitro diagnostic (IVD) use in USA

DescriptionCat. No.
0.1 ml, concentrate 202M-94
0.5 ml, concentrate 202M-95
1 ml, concentrate 202M-96
1 ml, predilute 202M-97
7 ml, predilute 202M-98
25 ml, predilute 202M-90

For in vitro diagnostic (IVD) use in Canada

DescriptionCat. No.
0.1 ml, concentrate 202M-94
0.5 ml, concentrate 202M-95
1 ml, concentrate 202M-96
1 ml, predilute 202M-97
7 ml, predilute 202M-98
25 ml, predilute 202M-90

For in vitro diagnostic (IVD) use in the EU

DescriptionCat. No.
0.1 ml, concentrate 202M-94
0.5 ml, concentrate 202M-95
1 ml, concentrate 202M-96
1 ml, predilute 202M-97
7 ml, predilute 202M-98
25 ml, predilute 202M-90

For research use only (RUO) in Japan

DescriptionCat. No.
0.1 ml, concentrate 202M-94 (RUO)
0.5 ml, concentrate 202M-95 (RUO)
1 ml, concentrate 202M-96 (RUO)
1 ml, predilute 202M-97 (RUO)
7 ml, predilute 202M-98 (RUO)
25 ml, predilute 202M-90 (RUO)

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: appendix, uterus, vessel wall
  • Isotype: IgG/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana ultraView
    UV/32min @ RT + AMP

Download an IFU

Select revision:


Keycode Specific IFU

Keycode:
Select prefix.
Please verify your keycode and try again. If problems presist contact Customer Service
Enter keycode.
Please verify your keycode and try again. If problems presist contact Customer Service

Some products may have special labeling requirements in the United States.

Are you located in the U.S.?


*Note: IFUs with the prefix RUO or ASR are only available for download in English.



Click here to learn about keycodes

References


  1. Cooke PH. J Cell Biol. 1976; 68:539-556.
  2. Skalli O, et al. J Cell Biol. 1986; 103:2787-2796.
  3. Gown AM, et al. J Cell Biol. 1985; 100:807- 813.
  4. Kuroda M. Biochem Biophys Acta. 1985; 843:20-213.
  5. Lazarides E. J Histochem Cytochem. 1975; 223:507-528.
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