Cell Marque Portfolio

Napsin A (MRQ-60)

Mouse Monoclonal


Napsin is a pepsin-like aspartic proteinase in the A1 clan of the AA clade of proteinases.1-3 There are two closely related napsins, napsin A (NAPSA) and napsin B (NAPSB).1-3 Napsin A is involved in processing propeptide pulmonary surfactant protein B (proSP-B) in the lung.4 In normal tissue, Napsin A is expressed in type II pneumocytes of the lung and proximal tubules of the kidney.1-3 Napsin A is a useful marker for lung adenocarcinoma.1-3, 5-8

Specialties: Cytopathology Pulmonary Pathology

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: lung adenocarcinoma, kidney, renal cell carcinoma
  • Isotype: IgG1/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana OptiView
    OV/CC1(32min)/8min @ 37°C
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

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References


  1. Jagirdar J. Application of immunohistochemistry to the diagnosis of primary and metastatic carcinoma to the lung. Arch Pathol Lab Med. 2008; 132:384-96.
  2. Bishop JA, et al. Napsin A and thyroid transcription factor-1 expression in carcinomas of the lung, breast, pancreas, colon, kidney, thyroid, and malignant mesothelioma. Hum Pathol. 2010; 41:20-5.
  3. Rawlings ND and Salvesen GS. Handbook of Proteolytic Enzymes Volume 1. 3rd Edition. Academic Press. 2013; p.69-71.
  4. Brasch F, et al. Involvement of napsin A in the C- and N-terminal processing of surfactant protein B in type-II pneumocytes of the human lung. J Biol Chem. 2003; 278: 49006-14.
  5. Dejmek A, et al. Napsin A (TA02) is a useful alternative to thyroid transcription factor-1 (TTF-1) for the identification of pulmonary adenocarcinoma cells in pleural effusions. Diagn Cytopathol. 2007; 35:493-7.
  6. Inamura K, et al. Pulmonary adenocarcinomas with enteric differentiation: histologic and immunohistochemical characteristics compared with metastatic colorectal cancers and usual pulmonary adenocarcinomas. Am J Surg Pathol. 2005; 29:660-5.
  7. Ye J, et al. Combination of Napsin A and TTF-1 immunohistochemistry helps in differentiating primary lung adenocarcinoma from metastatic carcinoma in the lung. Appl Immunohistochem Mol Morphol. 2011; 19:313-17.
  8. Mukhopadhyay S, et al. Subclassification of non-small cell lung carcinomas lacking morphologic differentiation on biopsy specimens: Utility of an immunohistochemical panel containing TTF-1, napsin A, p63, and CK5/6. Am J Surg Pathol. 2011; 35:15-25.

Last updated: 2017-03-23 11:44:27

Napsin A
Lung adenocarcinoma tumor cells are stained by anti-napsin A.

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Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: lung adenocarcinoma, kidney, renal cell carcinoma
  • Isotype: IgG1/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana OptiView
    OV/CC1(32min)/8min @ 37°C
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

References


  1. Jagirdar J. Application of immunohistochemistry to the diagnosis of primary and metastatic carcinoma to the lung. Arch Pathol Lab Med. 2008; 132:384-96.
  2. Bishop JA, et al. Napsin A and thyroid transcription factor-1 expression in carcinomas of the lung, breast, pancreas, colon, kidney, thyroid, and malignant mesothelioma. Hum Pathol. 2010; 41:20-5.
  3. Rawlings ND and Salvesen GS. Handbook of Proteolytic Enzymes Volume 1. 3rd Edition. Academic Press. 2013; p.69-71.
  4. Brasch F, et al. Involvement of napsin A in the C- and N-terminal processing of surfactant protein B in type-II pneumocytes of the human lung. J Biol Chem. 2003; 278: 49006-14.
  5. Dejmek A, et al. Napsin A (TA02) is a useful alternative to thyroid transcription factor-1 (TTF-1) for the identification of pulmonary adenocarcinoma cells in pleural effusions. Diagn Cytopathol. 2007; 35:493-7.
  6. Inamura K, et al. Pulmonary adenocarcinomas with enteric differentiation: histologic and immunohistochemical characteristics compared with metastatic colorectal cancers and usual pulmonary adenocarcinomas. Am J Surg Pathol. 2005; 29:660-5.
  7. Ye J, et al. Combination of Napsin A and TTF-1 immunohistochemistry helps in differentiating primary lung adenocarcinoma from metastatic carcinoma in the lung. Appl Immunohistochem Mol Morphol. 2011; 19:313-17.
  8. Mukhopadhyay S, et al. Subclassification of non-small cell lung carcinomas lacking morphologic differentiation on biopsy specimens: Utility of an immunohistochemical panel containing TTF-1, napsin A, p63, and CK5/6. Am J Surg Pathol. 2011; 35:15-25.

Download an IFU

Select revision:


Download SDS


Ordering Information

For in vitro diagnostic (IVD) use in USA

DescriptionCat. No.
0.1 ml, concentrate 352M-94
0.5 ml, concentrate 352M-95
1 ml, concentrate 352M-96
1 ml, predilute 352M-97
7 ml, predilute 352M-98
25 ml, predilute 352M-90

For in vitro diagnostic (IVD) use in Canada

DescriptionCat. No.
0.1 ml, concentrate 352M-94
0.5 ml, concentrate 352M-95
1 ml, concentrate 352M-96
1 ml, predilute 352M-97
7 ml, predilute 352M-98
25 ml, predilute 352M-90

For in vitro diagnostic (IVD) use in the EU

DescriptionCat. No.
0.1 ml, concentrate 352M-94
0.5 ml, concentrate 352M-95
1 ml, concentrate 352M-96
1 ml, predilute 352M-97
7 ml, predilute 352M-98
25 ml, predilute 352M-90

For research use only (RUO) in Japan

DescriptionCat. No.
0.1 ml, concentrate 352M-94-RUO
0.5 ml, concentrate 352M-95-RUO
1 ml, concentrate 352M-96-RUO
1 ml, predilute 352M-97-RUO
7 ml, predilute 352M-98-RUO
25 ml, predilute 352M-90-RUO

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic
  • Control: lung adenocarcinoma, kidney, renal cell carcinoma
  • Isotype: IgG1/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana OptiView
    OV/CC1(32min)/8min @ 37°C
  • Ventana ultraView
    UV/CC1M/16min @ 37°C
  • Leica Bond
    Protocol F: ER2/20

Download an IFU

Select revision:


Keycode Specific IFU

Keycode:
Select prefix.
Please verify your keycode and try again. If problems presist contact Customer Service
Enter keycode.
Please verify your keycode and try again. If problems presist contact Customer Service

Some products may have special labeling requirements in the United States.

Are you located in the U.S.?


*Note: IFUs with the prefix RUO or ASR are only available for download in English.



Click here to learn about keycodes

Download SDS

References


  1. Jagirdar J. Application of immunohistochemistry to the diagnosis of primary and metastatic carcinoma to the lung. Arch Pathol Lab Med. 2008; 132:384-96.
  2. Bishop JA, et al. Napsin A and thyroid transcription factor-1 expression in carcinomas of the lung, breast, pancreas, colon, kidney, thyroid, and malignant mesothelioma. Hum Pathol. 2010; 41:20-5.
  3. Rawlings ND and Salvesen GS. Handbook of Proteolytic Enzymes Volume 1. 3rd Edition. Academic Press. 2013; p.69-71.
  4. Brasch F, et al. Involvement of napsin A in the C- and N-terminal processing of surfactant protein B in type-II pneumocytes of the human lung. J Biol Chem. 2003; 278: 49006-14.
  5. Dejmek A, et al. Napsin A (TA02) is a useful alternative to thyroid transcription factor-1 (TTF-1) for the identification of pulmonary adenocarcinoma cells in pleural effusions. Diagn Cytopathol. 2007; 35:493-7.
  6. Inamura K, et al. Pulmonary adenocarcinomas with enteric differentiation: histologic and immunohistochemical characteristics compared with metastatic colorectal cancers and usual pulmonary adenocarcinomas. Am J Surg Pathol. 2005; 29:660-5.
  7. Ye J, et al. Combination of Napsin A and TTF-1 immunohistochemistry helps in differentiating primary lung adenocarcinoma from metastatic carcinoma in the lung. Appl Immunohistochem Mol Morphol. 2011; 19:313-17.
  8. Mukhopadhyay S, et al. Subclassification of non-small cell lung carcinomas lacking morphologic differentiation on biopsy specimens: Utility of an immunohistochemical panel containing TTF-1, napsin A, p63, and CK5/6. Am J Surg Pathol. 2011; 35:15-25.
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