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CD33 (PWS44)

Mouse Monoclonal


CD33, also known as gp67 or SIGLEC-3, is a 67 kDa glycosylated transmembrane protein that is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family.1-3 Although the precise physiological function of CD33 is unknown, it may mediate cell to cell adhesion and modulate inflammatory and immune response.1-3 In normal tissue, anti-CD33 labels myeloid cells (especially myeloid precursors), liver Kupffer cells, lung alveolar macrophages, and placental syncytiotrophoblasts.2,3 In neoplastic tissue, anti-CD33 is useful for the identification of acute myeloid leukemia.2,3

Specialties: Hematopathology

Specifications


  • Reactivity: paraffin
  • Visualization: membranous
  • Control: acute myeloid leukemia with monocytic differentiation or with minimal differentiation, placenta syncytiotrophoblasts
  • Isotype: IgG2b
  • Dilution Range: 1:20-1:80*

Protocols


  • Cell Marque HiDef
    T-PC/30-60minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/30-60minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1S/32min @ 37°C

Package Inserts


IFU SDS

References


  1. Freeman SD, et al. Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood. 1995; 85:2005-12.
  2. Hoyer JD, et al. CD33 detection by immunohistochemistry in paraffin-embedded tissues: a new antibody shows excellent specificity and sensitivity for cells of myelomonocytic lineage. Am J Clin Pathol. 2008; 129:316-23.
  3. Laszlo GS, et al. Expression and functional characterization of CD33 transcript variants in human acute myeloid leukemia. Oncotarget. 2016; 7:43281-94.

Last updated: 2017-05-24 08:46:09

CD33
CD33 mouse monoclonal antibody stains acute myelomonocytic leukemia strongly in a cytoplasmic pattern.

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Specifications


  • Reactivity: paraffin
  • Visualization: membranous
  • Control: acute myeloid leukemia with monocytic differentiation or with minimal differentiation, placenta syncytiotrophoblasts
  • Isotype: IgG2b
  • Dilution Range: 1:20-1:80*

Protocols


  • Cell Marque HiDef
    T-PC/30-60minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/30-60minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1S/32min @ 37°C

References


  1. Freeman SD, et al. Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood. 1995; 85:2005-12.
  2. Hoyer JD, et al. CD33 detection by immunohistochemistry in paraffin-embedded tissues: a new antibody shows excellent specificity and sensitivity for cells of myelomonocytic lineage. Am J Clin Pathol. 2008; 129:316-23.
  3. Laszlo GS, et al. Expression and functional characterization of CD33 transcript variants in human acute myeloid leukemia. Oncotarget. 2016; 7:43281-94.

Package Inserts

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Ordering Information

For in vitro diagnostic (IVD) use in USA

0.1 mL concentrate 133M-14
0.5 mL concentrate 133M-15
1 mL concentrate 133M-16
1 mL predilute 133M-17
7 mL predilute 133M-18

For in vitro diagnostic (IVD) use in Canada

0.1 mL concentrate 133M-14
0.5 mL concentrate 133M-15
1 mL concentrate 133M-16
1 mL predilute 133M-17
7 mL predilute 133M-18

For in vitro diagnostic (IVD) use in Europe

0.1 mL concentrate 133M-14
0.5 mL concentrate 133M-15
1 mL concentrate 133M-16
1 mL predilute 133M-17
7 mL predilute 133M-18

For research use only (RUO) in Japan

0.1 mL concentrate 133M-14-RUO
0.5 mL concentrate 133M-15-RUO
1 mL concentrate 133M-16-RUO
1 mL predilute 133M-17-RUO
7 mL predilute 133M-18-RUO

 

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Specifications


  • Reactivity: paraffin
  • Visualization: membranous
  • Control: acute myeloid leukemia with monocytic differentiation or with minimal differentiation, placenta syncytiotrophoblasts
  • Isotype: IgG2b
  • Dilution Range: 1:20-1:80*

Protocols


  • Cell Marque HiDef
    T-PC/30-60minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/30-60minAb/HiDef/DAB
  • Ventana ultraView
    UV/CC1S/32min @ 37°C

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References


  1. Freeman SD, et al. Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood. 1995; 85:2005-12.
  2. Hoyer JD, et al. CD33 detection by immunohistochemistry in paraffin-embedded tissues: a new antibody shows excellent specificity and sensitivity for cells of myelomonocytic lineage. Am J Clin Pathol. 2008; 129:316-23.
  3. Laszlo GS, et al. Expression and functional characterization of CD33 transcript variants in human acute myeloid leukemia. Oncotarget. 2016; 7:43281-94.
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