BOB.1 (MRQ-35) Mouse Monoclonal Antibody

BOB.1
Germinal center cells are moderately stained by BOB.1 antibody.

BOB.1 (MRQ-35) Mouse Monoclonal Antibody

Specialties: Hematopathology

Updated: 2017-05-24 08:46:09

Oct-binding factor-1 (OBF1), also known as BOB.1, is a B-cell-specific coactivator which has been mapped to chromosome 11q23.1 Expression of BOB.1/OBF.1 is restricted largely to mature B-cells, with germinal center B-cells normally staining for BOB.1.2,3 Analyses of BOB.1/OBF.1 expression in a variety of established B-cell lines representing different stages of B-cell development has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immunoreactive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immunoreactivity within the Hodgkin and Reed-Sternberg cells.3-7 Expression of BOB.1/OBF.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.8-12 B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.11-12

  1. Junker S, et al. Assignment of the human gene for Oct-Binding Factor-1 (OBF1), a B-Cell-spefific coactivator of Octamer-Binding transcription Factors1 and 2, to 11q23.1 by somatic cell hybridization and in situ hybridization. Genomics. 1996; 33:143-5.
  2. Dabbs DJ. Diagnostic Immunohistochemistry, Third Edition. Saunders. 2006.
  3. Steimle-Grauer SA, et al. Expression patterns of transcription factors in progressively transformed germinal centers and hodgkin lymphoma. Virchows Arch. 2003; 442:284-93.
  4. Valsami S, et al. A clinicopathological study of B-cell differentiation markers and transcription factors in classical hodgkin lymphoma: a potential prognostic role of mum1/IRF4. Haematologica. 2007; 92:1343-50.
  5. Stein H, et al. Down regulation of Bob.1 /OBF.1 and oct2 in classical Hodgkins disease but no in lymphocyte perdominant Hodgkins disease correlates with immunglobulin transcription. Blood. 2001; 97:496-501.
  6. Hertel CB, et al. Loss of B cell identity correlates with loss of B Cell-specific transcription factors in Hodgkin / Reed-Sternberg Cells of classical Hodgkin lymphoma. Oncogene. 2002; 21:4908-20.
  7. Pileri SA, et al. Primary mediastinal B_cell lymphoma: high frequence of BCL-6 mutations and consistent expression of the transcription factors OCT-2, Bob.1, PU.1 in the absence of immunoglobulins. Am J Pathology 2003; 162:243-53.
  8. Hoefnagel JJ, et al. Expression of B-cell transcription factors in primary cutaneous B-cell lymphoma. Moder Pathology. 2006; 19:1270-6.
  9. Greiner A, et al. Up-regulation of BOB.1/OBF.1 expression in normal germinal center B cells and germinal center-derived lymphomas. Am J Pathol. 2000; 156:501-7.
  10. Kuroda H, et al. Primary diffuse large B-cell lymphoma of the breast. Breast Cancer. 2007; 14:317-22.
  11. Sáez AI, et al. Analysis of octamer-binding transcription factors Oct2 and Oct1 and their coactivator BOB.1/OBF.1 in lymphomas. Mod Pathol. 2002; 15:211-20.
  12. Loddenkemper C, et al. Differential Emu enhancer activity and expression of BOB.1/OBF.1, Oct2, PU.1, and immunoglobulin in reactive B-cell populations, B-cell non-Hodgkin lymphomas, and Hodgkin lymphomas. J Pathol. 2004; 202:60-9.

Specifications


  • Reactivity: paraffin
  • Visualization: cytoplasmic, nuclear
  • Control: tonsil
  • Dilution Range: 1:5-1:25*

Package Inserts


IFU SDS

Ordering Information

For in vitro diagnostic (IVD) use in USA

0.1 mL concentrate294M-14
0.5 mL concentrate294M-15
1 mL concentrate294M-16
1 mL predilute294M-17
7 mL predilute294M-18

For in vitro diagnostic (IVD) use in Canada

0.1 mL concentrate294M-14
0.5 mL concentrate294M-15
1 mL concentrate294M-16
1 mL predilute294M-17
7 mL predilute294M-18

For in vitro diagnostic (IVD) use in Europe

0.1 mL concentrate294M-14
0.5 mL concentrate294M-15
1 mL concentrate294M-16
1 mL predilute294M-17
7 mL predilute294M-18

For research use only (RUO) in Japan

0.1 mL concentrate294M-14-RUO
0.5 mL concentrate294M-15-RUO
1 mL concentrate294M-16-RUO
1 mL predilute294M-17-RUO
7 mL predilute294M-18-RUO

 

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