Cell Marque Portfolio

WT1 (6F-H2)

Mouse Monoclonal


Wilms’ tumor 1 protein (WT1) is a zinc finger transcription factor, normally expressed in tissues of mesodermal origin.1 The Wilms’ tumor gene encodes a protein that functions as a tumor suppressor gene.2  WT1 is detected in tumor cells of Wilms’ Tumor (also  known as nephroblastoma) and mesothelioma.3 Additionally, WT1 expression has been found in ovarian serous carcinomas4 and some breast carcinomas.1

Specialties: Anatomic Pathology

Specifications


  • Reactivity: paraffin
  • Visualization: nuclear
  • Control: ovarian serous carcinoma, mesothelioma, kidney, testis
  • Isotype: IgG1/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana OptiView
    OV/CC1(32min)/32min @ 37°C
  • Ventana ultraView
    UV/CC1S/32min @ 37°C

References


  1. May RJ, et al. Peptide epitopes from the Wilms' tumor 1 oncoprotein stimulate CD4+ and CD8+ T cells that recognize and kill human malignant mesothelioma tumor cells. Clin Cancer Res. 2007; 13: 4547-55.
  2. Foster MR, et al. Immunohistochemical analysis of nuclear versus cytoplasmic staining of WT1 in malignant mesotheliomas and primary pulmonary adenocarcinomas. Arch Pathol Lab Med. 2001; 125:1316-20.
  3. Nakatsuka S, et al. Immunohistochemical detection of WT1 protein in a variety of cancer cells. Mod Pathol. 2006; 19:804-14.
  4. Goldstein NS, et al. WT1 immunoreactivity in uterine papillary serous carcinomas is different from ovarian serous carcinomas. Am J Clin Pathol. 2002; 117:541-5.

Literature

Last updated: 2017-05-24 08:46:09

WT1
Nuclear labelling of cells of mesothelioma by WT1 antibody.

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Specifications


  • Reactivity: paraffin
  • Visualization: nuclear
  • Control: ovarian serous carcinoma, mesothelioma, kidney, testis
  • Isotype: IgG1/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana OptiView
    OV/CC1(32min)/32min @ 37°C
  • Ventana ultraView
    UV/CC1S/32min @ 37°C

References


  1. May RJ, et al. Peptide epitopes from the Wilms' tumor 1 oncoprotein stimulate CD4+ and CD8+ T cells that recognize and kill human malignant mesothelioma tumor cells. Clin Cancer Res. 2007; 13: 4547-55.
  2. Foster MR, et al. Immunohistochemical analysis of nuclear versus cytoplasmic staining of WT1 in malignant mesotheliomas and primary pulmonary adenocarcinomas. Arch Pathol Lab Med. 2001; 125:1316-20.
  3. Nakatsuka S, et al. Immunohistochemical detection of WT1 protein in a variety of cancer cells. Mod Pathol. 2006; 19:804-14.
  4. Goldstein NS, et al. WT1 immunoreactivity in uterine papillary serous carcinomas is different from ovarian serous carcinomas. Am J Clin Pathol. 2002; 117:541-5.

Package Inserts

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Ordering Information

For in vitro diagnostic (IVD) use in USA

0.1 mL concentrate 348M-94
0.5 mL concentrate 348M-95
1 mL concentrate 348M-96
1 mL predilute 348M-97
7 mL predilute 348M-98
25 mL predilute 348M-90

For in vitro diagnostic (IVD) use in Canada

0.1 mL concentrate 348M-94
0.5 mL concentrate 348M-95
1 mL concentrate 348M-96
1 mL predilute 348M-97
7 mL predilute 348M-98
25 mL predilute 348M-90

For in vitro diagnostic (IVD) use in the EU

0.1 mL concentrate 348M-94
0.5 mL concentrate 348M-95
1 mL concentrate 348M-96
1 mL predilute 348M-97
7 mL predilute 348M-98
25 mL predilute 348M-90

For research use only (RUO) in Japan

0.1 mL concentrate 348M-94-RUO
0.5 mL concentrate 348M-95-RUO
1 mL concentrate 348M-96-RUO
1 mL predilute 348M-97-RUO
7 mL predilute 348M-98-RUO
25 mL predilute 348M-90-RUO

Literature

Specifications


  • Reactivity: paraffin
  • Visualization: nuclear
  • Control: ovarian serous carcinoma, mesothelioma, kidney, testis
  • Isotype: IgG1/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana OptiView
    OV/CC1(32min)/32min @ 37°C
  • Ventana ultraView
    UV/CC1S/32min @ 37°C

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References


  1. May RJ, et al. Peptide epitopes from the Wilms' tumor 1 oncoprotein stimulate CD4+ and CD8+ T cells that recognize and kill human malignant mesothelioma tumor cells. Clin Cancer Res. 2007; 13: 4547-55.
  2. Foster MR, et al. Immunohistochemical analysis of nuclear versus cytoplasmic staining of WT1 in malignant mesotheliomas and primary pulmonary adenocarcinomas. Arch Pathol Lab Med. 2001; 125:1316-20.
  3. Nakatsuka S, et al. Immunohistochemical detection of WT1 protein in a variety of cancer cells. Mod Pathol. 2006; 19:804-14.
  4. Goldstein NS, et al. WT1 immunoreactivity in uterine papillary serous carcinomas is different from ovarian serous carcinomas. Am J Clin Pathol. 2002; 117:541-5.
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