Cell Marque Portfolio

WT1 (6F-H2)

Mouse Monoclonal


Anti-WT1 is useful in the differential diagnosis of pulmonary malignancies in that up to 93% of malignant mesotheliomas (nuclei) are labeled with this antibody.  In contrast, the nuclei of lung adenocarcinomas are not labeled with this product (0 of 50 in a recent study).1-5   However, cytoplasmic labeling of lung adenocarcinomas may be observed.  The differential diagnosis of abdominal papillary carcinomas can be problematic.   Anti-WT1 labels 93% of serous ovarian carcinomas and 0% (nuclei) of mucinous carcinomas of the ovary and pancreato-biliary carcinomas.Anti-WT1 can also be utilized in the differential diagnosis of small round cell tumors as 100% of desmoplastic small round cell tumors and 70% of nephroblastomas (Wilm’s tumor) are positive (nuclei) for this marker. Tumors such as Ewings sarcoma/PNET, neuroblastomas, rhabdomyosarcomas, and rhabdoid tumors are negative.  Occasionally cytoplasmic labeling may be seen in these tumors, however.7

Specialties: Anatomic Pathology

Specifications


  • Reactivity: paraffin
  • Visualization: nuclear
  • Control: ovarian serous carcinoma, mesothelioma, kidney, testis
  • Isotype: IgG1/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana OptiView
    OV/CC1(32min)/32min @ 37°C
  • Ventana ultraView
    UV/CC1S/32min @ 37°C

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References


  1. Tsuta K, et al. Appl Immunohistochem Mol Morphol. 2009; 17:126-130.
  2. Marchevsky AM. Arch Pathol Lab Med. 2008; 132:397-401.
  3. Ordonez NG. Am J Surg Pathol. 1998; 22:1203-1214.
  4. Ordonez NG. Arch Pathol Lab Med. 2005; 129:1407-1414.
  5. Yaziji H, et al. Mod Pathol. 2006; 19:514-523.
  6. Goldstein NS, et al. Am J Clin Pathol. 2002; 117:541-545.
  7. Barnoud R, et al. Am J Surg Pathol. 2000; 24:830-836.

Literature

Last updated: 2016-09-29 13:18:40

WT1
Nuclear labelling of cells of mesothelioma by WT1 antibody.

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Specifications


  • Reactivity: paraffin
  • Visualization: nuclear
  • Control: ovarian serous carcinoma, mesothelioma, kidney, testis
  • Isotype: IgG1/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana OptiView
    OV/CC1(32min)/32min @ 37°C
  • Ventana ultraView
    UV/CC1S/32min @ 37°C

References


  1. Tsuta K, et al. Appl Immunohistochem Mol Morphol. 2009; 17:126-130.
  2. Marchevsky AM. Arch Pathol Lab Med. 2008; 132:397-401.
  3. Ordonez NG. Am J Surg Pathol. 1998; 22:1203-1214.
  4. Ordonez NG. Arch Pathol Lab Med. 2005; 129:1407-1414.
  5. Yaziji H, et al. Mod Pathol. 2006; 19:514-523.
  6. Goldstein NS, et al. Am J Clin Pathol. 2002; 117:541-545.
  7. Barnoud R, et al. Am J Surg Pathol. 2000; 24:830-836.

Download an IFU

Select revision:



Ordering Information

For in vitro diagnostic (IVD) use in USA

DescriptionCat. No.
0.1 ml, concentrate 348M-94
0.5 ml, concentrate 348M-95
1 ml, concentrate 348M-96
1 ml, predilute 348M-97
7 ml, predilute 348M-98
25 ml, predilute 348M-90

For in vitro diagnostic (IVD) use in Canada

DescriptionCat. No.
0.1 ml, concentrate 348M-94
0.5 ml, concentrate 348M-95
1 ml, concentrate 348M-96
1 ml, predilute 348M-97
7 ml, predilute 348M-98
25 ml, predilute 348M-90

For in vitro diagnostic (IVD) use in the EU

DescriptionCat. No.
0.1 ml, concentrate 348M-94
0.5 ml, concentrate 348M-95
1 ml, concentrate 348M-96
1 ml, predilute 348M-97
7 ml, predilute 348M-98
25 ml, predilute 348M-90

For research use only (RUO) in Japan

DescriptionCat. No.
0.1 ml, concentrate 348M-94 (RUO)
0.5 ml, concentrate 348M-95 (RUO)
1 ml, concentrate 348M-96 (RUO)
1 ml, predilute 348M-97 (RUO)
7 ml, predilute 348M-98 (RUO)
25 ml, predilute 348M-90 (RUO)

Literature

Specifications


  • Reactivity: paraffin
  • Visualization: nuclear
  • Control: ovarian serous carcinoma, mesothelioma, kidney, testis
  • Isotype: IgG1/k
  • Dilution Range: 1:100-1:500*

Protocols


  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/Perm Red
  • Cell Marque HiDef
    T-PC/10-30minAb/HiDef/DAB
  • Ventana OptiView
    OV/CC1(32min)/32min @ 37°C
  • Ventana ultraView
    UV/CC1S/32min @ 37°C

Download an IFU

Select revision:


Keycode Specific IFU

Keycode:
Select prefix.
Please verify your keycode and try again. If problems presist contact Customer Service
Enter keycode.
Please verify your keycode and try again. If problems presist contact Customer Service

Some products may have special labeling requirements in the United States.

Are you located in the U.S.?


*Note: IFUs with the prefix RUO or ASR are only available for download in English.



Click here to learn about keycodes

References


  1. Tsuta K, et al. Appl Immunohistochem Mol Morphol. 2009; 17:126-130.
  2. Marchevsky AM. Arch Pathol Lab Med. 2008; 132:397-401.
  3. Ordonez NG. Am J Surg Pathol. 1998; 22:1203-1214.
  4. Ordonez NG. Arch Pathol Lab Med. 2005; 129:1407-1414.
  5. Yaziji H, et al. Mod Pathol. 2006; 19:514-523.
  6. Goldstein NS, et al. Am J Clin Pathol. 2002; 117:541-545.
  7. Barnoud R, et al. Am J Surg Pathol. 2000; 24:830-836.
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